Differences in the antibody response to adult Fasciola hepatica excretory/secretory products in experimentally and naturally infected cattle and sheep



Walsh, Tessa R, Ainsworth, Stuart, Armstrong, Stuart ORCID: 0000-0002-3862-1801, Hodgkinson, Jane ORCID: 0000-0001-9152-8481 and Williams, Diana ORCID: 0000-0001-8186-7236
(2020) Differences in the antibody response to adult Fasciola hepatica excretory/secretory products in experimentally and naturally infected cattle and sheep. Veterinary Parasitology, 289. p. 109321.

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Abstract

Fasciola hepatica (the liver fluke) is a common, global parasite of livestock. It can be highly pathogenic and has health and welfare implications for infected individuals. Typically, in ruminants, infections are sub-clinical, but if undiagnosed, they can lead to significant production losses. Accurate diagnosis is crucial to identify infection. Antibody detection ELISAs are commonly used to diagnose infection due to their high sensitivity and specificity and are typically based on native fluke excretory/secretory (ES) products or cathepsin L1 (CL1), the immunodominant antigen within ES products. These tests have been developed based on the antibody response of experimentally infected animals; however, this response has not been well characterised in naturally infected animals. We compared the antibody recognition of a recombinant CL1 (rCL1) antigen and native adult fluke ES products. Whilst samples from experimentally infected animals showed strong recognition of rCL1, serum antibodies from naturally infected animals did not. These results were confirmed by peptide array. Immunoblotting sera against ES products showed that experimentally infected animals had a strong, specific response to CL1/CL2 proteins whilst antibodies from naturally infected animals recognised multiple proteins and had a variable response to CL1/CL2. Mass spectrometry of proteins separated by 2D SDS PAGE, identified several antigens recognised by serum antibodies from a naturally infected cow, including cathepsins L1, L2 and L5, glutathione S-transferase and a dihydrolipoyl dehydrogenase. Overall, these results show that the antibody response in naturally infected animals to adult fluke ES products is qualitatively different to experimentally infected animals. This suggests that a diagnostic test based on CL1 alone may not be appropriate for diagnosis of natural F. hepatica infections in sheep and cattle.

Item Type: Article
Uncontrolled Keywords: Fasciola hepatica, Liver fluke, Cathepsin L1, Serodiagnosis, Antibody response
Depositing User: Symplectic Admin
Date Deposited: 20 Nov 2020 08:50
Last Modified: 18 Jan 2023 23:20
DOI: 10.1016/j.vetpar.2020.109321
Open Access URL: https://www.sciencedirect.com/science/article/pii/...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3107543