Ryan, Kathryn A, Bewley, Kevin R, Fotheringham, Susan A, Slack, Gillian S, Brown, Phillip, Hall, Yper, Wand, Nadina I, Marriott, Anthony C, Cavell, Breeze E, Tree, Julia A et al (show 45 more authors)
(2021)
Dose-dependent response to infection with SARS-CoV-2 in the ferret model and evidence of protective immunity.
NATURE COMMUNICATIONS, 12 (1).
81-.
Abstract
There is a vital need for authentic COVID-19 animal models to enable the pre-clinical evaluation of candidate vaccines and therapeutics. Here we report a dose titration study of SARS-CoV-2 in the ferret model. After a high (5 × 10<sup>6</sup> pfu) and medium (5 × 10<sup>4</sup> pfu) dose of virus is delivered, intranasally, viral RNA shedding in the upper respiratory tract (URT) is observed in 6/6 animals, however, only 1/6 ferrets show similar signs after low dose (5 × 10<sup>2</sup> pfu) challenge. Following sequential culls pathological signs of mild multifocal bronchopneumonia in approximately 5-15% of the lung is seen on day 3, in high and medium dosed groups. Ferrets re-challenged, after virus shedding ceased, are fully protected from acute lung pathology. The endpoints of URT viral RNA replication & distinct lung pathology are observed most consistently in the high dose group. This ferret model of SARS-CoV-2 infection presents a mild clinical disease.
Item Type: | Article |
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Uncontrolled Keywords: | Lung, Animals, Ferrets, Disease Models, Animal, RNA, Viral, Antibodies, Viral, Virus Replication, Virus Shedding, Dose-Response Relationship, Drug, Female, COVID-19, SARS-CoV-2, COVID-19 Vaccines |
Depositing User: | Symplectic Admin |
Date Deposited: | 15 Jan 2021 09:21 |
Last Modified: | 18 Jan 2023 23:03 |
DOI: | 10.1038/s41467-020-20439-y |
Open Access URL: | http://doi.org/10.1038/s41467-020-20439-y |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3113641 |