Mwesigwa, Savannah, Williams, Lesedi, Retshabile, Gaone, Katagirya, Eric, Mboowa, Gerald, Mlotshwa, Busisiwe, Kyobe, Samuel, Kateete, David P, Wampande, Eddie Mujjwiga, Wayengera, Misaki et al (show 16 more authors)
(2021)
Unmapped exome reads implicate a role for <i>Anelloviridae</i> in childhood HIV-1 long-term non-progression.
NPJ GENOMIC MEDICINE, 6 (1).
24-.
Abstract
Human immunodeficiency virus (HIV) infection remains a significant public health burden globally. The role of viral co-infection in the rate of progression of HIV infection has been suggested but not empirically tested, particularly among children. We extracted and classified 42 viral species from whole-exome sequencing (WES) data of 813 HIV-infected children in Botswana and Uganda categorised as either long-term non-progressors (LTNPs) or rapid progressors (RPs). The Ugandan participants had a higher viral community diversity index compared to Batswana (p = 4.6 × 10<sup>-13</sup>), and viral sequences were more frequently detected among LTNPs than RPs (24% vs 16%; p = 0.008; OR, 1.9; 95% CI, 1.6-2.3), with Anelloviridae showing strong association with LTNP status (p = 3 × 10<sup>-4</sup>; q = 0.004, OR, 3.99; 95% CI, 1.74-10.25). This trend was still evident when stratified by country, sex, and sequencing platform, and after a logistic regression analysis adjusting for age, sex, country, and the sequencing platform (p = 0.02; q = 0.03; OR, 7.3; 95% CI, 1.6-40.5). Torque teno virus (TTV), which made up 95% of the Anelloviridae reads, has been associated with reduced immune activation. We identify an association between viral co-infection and prolonged AIDs-free survival status that may have utility as a biomarker of LTNP and could provide mechanistic insights to HIV progression in children, demonstrating the added value of interrogating off-target WES reads in cohort studies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Collaborative African Genomics Network (CAfGEN), TrypanoGEN Research Group |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 25 Mar 2021 08:21 |
| Last Modified: | 18 Oct 2023 17:06 |
| DOI: | 10.1038/s41525-021-00185-w |
| Open Access URL: | http://doi.org/10.1038/s41525-021-00185-w |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3118131 |
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