Anterior Gradient Protein 3 and S100 Calcium-Binding Protein P Levels in Different Endometrial Epithelial Compartments May Play an Important Role in Recurrent Pregnancy Failure

Tempest, Nicola ORCID: 0000-0003-3633-1592, Batchelor, Elizabeth, Hill, Christopher J ORCID: 0000-0003-3831-4569, Al-Lamee, Hannan, Drury, Josephine ORCID: 0000-0002-2275-6664, Drakeley, Andrew J and Hapangama, Dharani K ORCID: 0000-0003-0270-0150 (2021) Anterior Gradient Protein 3 and S100 Calcium-Binding Protein P Levels in Different Endometrial Epithelial Compartments May Play an Important Role in Recurrent Pregnancy Failure. International Journal of Molecular Sciences, 22 (8). p. 3835.

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Official URL: http://dx.doi.org/10.3390/ijms22083835

Abstract

<jats:p>Recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) are distressing conditions without effective treatments. The luminal epithelium (LE) is integral in determining receptivity of the endometrium, whereas functionalis glands and stroma aid in nurturing early embryo development. Calcium signalling pathways are known to be of vital importance to embryo implantation and pregnancy establishment, and anterior gradient protein 3 (AGR3) and S100 calcium-binding protein P (S100P) are involved with these pathways. We initially examined 20 full-thickness endometrial biopsies from premenopausal women across the menstrual cycle to characterize levels of AGR3 protein in each endometrial sub-region at the cellular level. A further 53 endometrial pipelle biopsies collected in the window of implantation were subsequently assessed to determine differential endometrial AGR3 and S100P levels relevant to RIF (n = 13) and RPL (n = 10) in comparison with parous women (n = 30) using immunohistochemistry. Significantly higher AGR3 and S100P immunostaining was observed in ciliated cells of the LE of women with recurrent reproductive failure compared with parous women, suggesting aberrant subcellular location-associated pathophysiology for these conditions. The nuclear localisation of S100P may allow transcriptional regulatory function, which is necessary for implantation of a viable pregnancy. Further work is thus warranted to assess their utility as diagnostic/therapeutic targets.</jats:p>

Item Type:	Article
Uncontrolled Keywords:	AGR3, S100P, fertility, reproduction, window of implantation
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Clinical Directorate Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	22 Apr 2021 10:33
Last Modified:	18 Jan 2023 22:51
DOI:	10.3390/ijms22083835
Open Access URL:	https://www.mdpi.com/1422-0067/22/8/3835
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3120174