Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity (vol 478, pg 735, 2021)



Byrne, Dominic P, London, James A ORCID: 0000-0002-5823-7057, Eyers, Patrick A ORCID: 0000-0002-9220-2966, Yates, Edwin A ORCID: 0000-0001-9365-5433 and Cartmell, Alan ORCID: 0000-0002-5512-249X
(2021) Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity (vol 478, pg 735, 2021). BIOCHEMICAL JOURNAL, 478 (1). pp. 2537-2538.

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Abstract

The authors would like to make the following corrections to their article. The equation appearing in the section 'A novel microfuidic-based desulfation assay' of the Methods, should appear as V0=(kcat/KM)[S][E]. The y-axis in Figure 2H should be 'Integral'. The corrected version can be seen below. The footnote in Table 1 should read "The table list the kinetic values and the corresponding amount of resources required in terms of time and components.*Indicates value is a specific activity (h-1). All experiments represent technical triplicates."The authors apologise for any confusion caused by these errors.

Item Type: Article
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 13 Jul 2021 07:18
Last Modified: 18 Jan 2023 21:36
DOI: 10.1042/BCJ20200952_COR
Open Access URL: https://portlandpress.com/biochemj/article/478/4/7...
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3129852