Theron, Chrispian W 
ORCID: 0000-0002-8522-6417, Vandermies, Marie, Telek, Samuel, Steels, Sebastien and Fickers, Patrick
(2020)
Comprehensive comparison of <i>Yarrowia lipolytica</i> and <i>Pichia pastoris</i> for production of <i>Candida antarctica</i> lipase B.
SCIENTIFIC REPORTS, 10 (1).
1741-.
Abstract
The large-scale production of recombinant proteins (rProt) is becoming increasingly economically important. Among the different hosts used for rProt production, yeasts are gaining popularity. The so-called non-conventional yeasts, such as the methylotrophic Pichia pastoris and the dimorphic Yarrowia lipolytica, are popular choices due to their favorable characteristics and well-established expression systems. Nevertheless, a direct comparison of the two systems for rProt production and secretion was lacking. This study therefore aimed to directly compare Y. lipolytica and P. pastoris for the production and secretion of lipase CalB in bioreactor. Y. lipolytica produced more than double the biomass and more than 5-fold higher extracellular lipase than P. pastoris. Furthermore, maximal CalB production levels were reached by Y. lipolytica in half the cultivation time required for maximal production by P. pastoris. Conversely, P. pastoris was found to express 7-fold higher levels of CalB mRNA. Secreted enhanced green fluorescent protein -in isolation and fused to CalB- and protease inhibitor MG-132 were used in P. pastoris to further investigate the reasons behind such discrepancy. The most likely explanation was ultimately found to be protein degradation by endoplasmic reticulum-associated protein degradation preceding successful secretion. This study highlighted the multifaceted nature of rProt production, prompting a global outlook for selection of rProt production systems.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Pichia, Yarrowia, Lipase, Fungal Proteins, Recombinant Proteins, Cloning, Molecular, Biomass |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 Jul 2021 08:16 |
| Last Modified: | 07 Oct 2023 05:45 |
| DOI: | 10.1038/s41598-020-58683-3 |
| Open Access URL: | http://doi.org/10.1038/s41598-020-58683-3 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3130242 |
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