One-step preparation of antimicrobial silicone materials based on PDMS and salicylic acid: insights from spatially and temporally resolved techniques

Barbieri, Luca, Sorzabal Bellido, Ioritz ORCID: 0000-0001-8050-1443, Beckett, Alison J ORCID: 0000-0001-8377-325X, Prior, Ian A ORCID: 0000-0002-4055-5161, Fothergill, Jo ORCID: 0000-0002-7012-1508, Diaz Fernandez, Yuri A ORCID: 0000-0003-3422-8663 and Raval, Rasmita (2021) One-step preparation of antimicrobial silicone materials based on PDMS and salicylic acid: insights from spatially and temporally resolved techniques. NPJ BIOFILMS AND MICROBIOMES, 7 (1). 51-.

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Abstract

In this work, we introduce a one-step strategy that is suitable for continuous flow manufacturing of antimicrobial PDMS materials. The process is based on the intrinsic capacity of PDMS to react to certain organic solvents, which enables the incorporation of antimicrobial actives such as salicylic acid (SA), which has been approved for use in humans within pharmaceutical products. By combining different spectroscopic and imaging techniques, we show that the surface properties of PDMS remain unaffected while high doses of the SA are loaded inside the PDMS matrix. The SA can be subsequently released under physiological conditions, delivering a strong antibacterial activity. Furthermore, encapsulation of SA inside the PDMS matrix ensured a diffusion-controlled release that was tracked by spatially resolved Raman spectroscopy, Attenuated Total Reflectance IR (ATR-IR), and UV-Vis spectroscopy. The biological activity of the new material was evaluated directly at the surface and in the planktonic state against model pathogenic bacteria, combining confocal laser scanning microscopy, electron microscopy, and cell viability assays. The results showed complete planktonic inhibition for clinically relevant strains of Staphylococcus aureus and Escherichia coli, and a reduction of up to 4 orders of magnitude for viable sessile cells, demonstrating the efficacy of these surfaces in preventing the initial stages of biofilm formation. Our approach adds a new option to existing strategies for the antimicrobial functionalisation of a wide range of products such as catheters, wound dressings and in-dwelling medical devices based on PDMS.

Item Type:	Article
Uncontrolled Keywords:	Humans, Salicylic Acid, Silicones, Dimethylpolysiloxanes, Nylons, Anti-Bacterial Agents, Spectrum Analysis, Microbial Sensitivity Tests, Molecular Structure, Surface Properties, Chemistry Techniques, Synthetic, Drug Liberation
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Tech, Infrastructure and Environmental Directorate Faculty of Science and Engineering > School of Physical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	05 Aug 2021 10:39
Last Modified:	18 Jan 2023 21:34
DOI:	10.1038/s41522-021-00223-6
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3132470