Salzer, Ralf, Clark, Jordan J, Vaysburd, Marina, Chang, Veronica T, Albecka, Anna, Kiss, Leo, Sharma, Parul, Llamazares, Andres Gonzalez, Kipar, Anja 
ORCID: 0000-0001-7289-3459, Hiscox, Julian A ORCID: 0000-0002-6582-0275 et al (show 5 more authors)
(2021)
Single-dose immunisation with a multimerised SARS-CoV-2 receptor binding domain (RBD) induces an enhanced and protective response in mice.
FEBS LETTERS, 595 (18).
pp. 2323-2340.
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Single-dose immunisation with a multimerised SARS-CoV-2 receptor binding domain (RBD) induces an enhanced and protective res.pdf - Published version Download (1MB) | Preview |
Abstract
The COVID-19 pandemic, caused by the SARS-CoV-2 coronavirus, has triggered a worldwide health emergency. Here, we show that ferritin-like Dps from hyperthermophilic Sulfolobus islandicus, covalently coupled with SARS-CoV-2 antigens via the SpyCatcher system, forms stable multivalent dodecameric vaccine nanoparticles that remain intact even after lyophilisation. Immunisation experiments in mice demonstrated that the SARS-CoV-2 receptor binding domain (RBD) coupled to Dps (RBD-S-Dps) elicited a higher antibody titre and an enhanced neutralising antibody response compared to monomeric RBD. A single immunisation with RBD-S-Dps completely protected hACE2-expressing mice from serious illness and led to viral clearance from the lungs upon SARS-CoV-2 infection. Our data highlight that multimerised SARS-CoV-2 subunit vaccines are a highly efficacious modality, particularly when combined with an ultra-stable scaffold.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | coronavirus, COVID-19, Dps, RBD, SARS-CoV-2, subunit vaccine |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 30 Sep 2021 10:51 |
| Last Modified: | 18 Jan 2023 21:27 |
| DOI: | 10.1002/1873-3468.14171 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3138811 |
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