Prophages encode phage-defense systems with cognate self-immunity



Owen, Sian V, Wenner, Nicolas, Dulberger, Charles L, Rodwell, Ella V, Bowers-Barnard, Arthur, Quinones-Olvera, Natalia, Rigden, Daniel J ORCID: 0000-0002-7565-8937, Rubin, Eric J, Garner, Ethan C, Baym, Michael
et al (show 1 more authors) (2021) Prophages encode phage-defense systems with cognate self-immunity. CELL HOST & MICROBE, 29 (11). 1620-+.

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Abstract

Temperate phages are pervasive in bacterial genomes, existing as vertically inherited islands termed prophages. Prophages are vulnerable to predation of their host bacterium by exogenous phages. Here, we identify BstA, a family of prophage-encoded phage-defense proteins in diverse Gram-negative bacteria. BstA localizes to sites of exogenous phage DNA replication and mediates abortive infection, suppressing the competing phage epidemic. During lytic replication, the BstA-encoding prophage is not itself inhibited by BstA due to self-immunity conferred by the anti-BstA (aba) element, a short stretch of DNA within the bstA locus. Inhibition of phage replication by distinct BstA proteins from Salmonella, Klebsiella, and Escherichia prophages is generally interchangeable, but each possesses a cognate aba element. The specificity of the aba element ensures that immunity is exclusive to the replicating prophage, preventing exploitation by variant BstA-encoding phages. The BstA protein allows prophages to defend host cells against exogenous phage attack without sacrificing the ability to replicate lytically.

Item Type: Article
Additional Information: I have a PDF of the acceptance email from the journal, but I was not sure where to upload this? Perhaps the acceptance email is no longer required by Elements?
Uncontrolled Keywords: Salmonella, Bacteriophages, Prophages, Genome, Bacterial
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 05 Oct 2021 15:52
Last Modified: 18 Jan 2023 21:27
DOI: 10.1016/j.chom.2021.09.002
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3139376

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