Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma

Rothzerg, Emel, Ho, Xuan Dung, Xu, Jiake, Wood, David, Maertson, Aare and Koks, Sulev ORCID: 0000-0001-6087-6643
(2021) Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma. GENES, 12 (8). 1132-.

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The human genome encodes thousands of natural antisense long noncoding RNAs (lncRNAs); they play the essential role in regulation of gene expression at multiple levels, including replication, transcription and translation. Dysregulation of antisense lncRNAs plays indispensable roles in numerous biological progress, such as tumour progression, metastasis and resistance to therapeutic agents. To date, there have been several studies analysing antisense lncRNAs expression profiles in cancer, but not enough to highlight the complexity of the disease. In this study, we investigated the expression patterns of antisense lncRNAs from osteosarcoma and healthy bone samples (24 tumour-16 bone samples) using RNA sequencing. We identified 15 antisense lncRNAs (<i>RUSC1-AS1</i>, <i>TBX2-AS1</i>, <i>PTOV1-AS1</i>, <i>UBE2D3-AS1</i>, <i>ERCC8-AS1</i>, <i>ZMIZ1-AS1</i>, <i>RNF144A-AS1</i>, <i>RDH10-AS1</i>, <i>TRG-AS1</i>, <i>GSN-AS1</i>, <i>HMGA2-AS1</i>, <i>ZNF528-AS1</i>, <i>OTUD6B-AS1</i>, <i>COX10-AS1</i> and <i>SLC16A1-AS1</i>) that were upregulated in tumour samples compared to bone sample controls. Further, we performed real-time polymerase chain reaction (RT-qPCR) to validate the expressions of the antisense lncRNAs in 8 different osteosarcoma cell lines (SaOS-2, G-292, HOS, U2-OS, 143B, SJSA-1, MG-63, and MNNG/HOS) compared to hFOB (human osteoblast cell line). These differentially expressed IncRNAs can be considered biomarkers and potential therapeutic targets for osteosarcoma.

Item Type: Article
Uncontrolled Keywords: osteosarcoma, sarcoma, non-coding RNA, antisense RNA, alternative splicing
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 28 Jan 2022 09:32
Last Modified: 10 Feb 2024 03:04
DOI: 10.3390/genes12081132
Open Access URL: https://doi.org/10.3390/genes12081132
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3147759