Transcriptional Organization of the <i>Salmonella</i> Typhimurium Phage P22 <i>pid</i> ORFan Locus

Wolput, Sanne, Makumi, Angela, Wicke, Laura, Baecker, Leonard E, Cenens, William, Briers, Yves, Wenner, Nicolas A, Owen, Sian V, Hinton, Jay CD, Lavigne, Rob
et al (show 1 more authors) (2022) Transcriptional Organization of the <i>Salmonella</i> Typhimurium Phage P22 <i>pid</i> ORFan Locus. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (3). 1253-.

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Many phage genes lack sequence similarity to any other open reading frame (ORF) in current databases. These enigmatic ORFan genes can have a tremendous impact on phage propagation and host interactions but often remain experimentally unexplored. We previously revealed a novel interaction between phage P22 and its <i>Salmonella</i> Typhimurium host, instigated by the ORFan gene <i>pid</i> (for phage P22 encoded instigator of <i>dgo</i> expression) and resulting in derepression of the host <i>dgoRKAT</i> operon. The <i>pid</i> gene is highly expressed in phage carrier cells that harbor a polarly located P22 episome that segregates asymmetrically among daughter cells. Here, we discovered that the <i>pid</i> locus is fitted with a weak promoter, has an exceptionally long 5' untranslated region that is instructive for a secondary <i>pid</i> mRNA species, and has a 3' Rho-independent termination loop that is responsible for stability of the <i>pid</i> transcript.

Item Type: Article
Uncontrolled Keywords: phage-host interactions, Salmonella Typhimurium, phage P22, pid ORFan locus, dgoRKAT operon
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 31 Jan 2022 16:45
Last Modified: 24 Jan 2024 17:54
DOI: 10.3390/ijms23031253
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