Romano, Vito, Parekh, Mohit, Kazaili, Ahmed, Steger, Bernhard, Akhtar, Riaz ORCID: 0000-0002-7963-6874, Ferrari, Stefano, Kaye, Stephen B ORCID: 0000-0003-0390-0592 and Levis, Hannah J ORCID: 0000-0002-3923-1178
(2022)
Eye bank versus surgeon prepared Descemet stripping automated endothelial keratoplasty tissues: Influence on adhesion force in a pilot study.
INDIAN JOURNAL OF OPHTHALMOLOGY, 70 (2).
pp. 523-528.
Text
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Abstract
<h4>Purpose</h4>To evaluate and compare the biomechanical properties of the eye bank-prepared and surgeon prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues.<h4>Methods</h4>In this laboratory study, corneal tissues for research were randomly allocated in the following groups: a) surgeon-cut DSAEK and b) eye bank-prepared (pre-cut and pre-loaded) DSAEK. Endothelial cell loss (ECL), immunostaining for tight junction protein ZO-1, elastic modulus, and adhesion force were investigated.<h4>Results</h4>ECL was not found to be significantly different between surgeon-cut DSAEK (7.8% ±6.5%), pre-cut DSAEK (8.6% ±2.3%), and pre-loaded DSAEK (11.1% ±4.8%) (P = 0.5910). ZO-1 was expressed equally across all groups. Surgeon-cut DSAEK grafts showed a significantly higher elastic modulus compared to pre-cut and pre-loaded DSAEK groups (P = 0.0047 and P < 0.0001, respectively). Adhesion force was significantly greater in the surgeon-cut DSAEK compared to pre-cut (P < 0.0001) or pre-loaded DSAEK groups (P = 0.0101).<h4>Conclusion</h4>The laboratory data on the biomechanics of DSAEK grafts suggests that surgeon-cut DSAEK grafts present higher elastic modulus and adhesion force compared to eye bank-prepared DSAEK grafts.
Item Type: | Article |
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Uncontrolled Keywords: | Adhesion, DSAEK, elastic modulus, pre-loaded, pre-stripped |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Science and Engineering > School of Engineering |
Depositing User: | Symplectic Admin |
Date Deposited: | 07 Mar 2022 10:12 |
Last Modified: | 18 Jan 2023 21:11 |
DOI: | 10.4103/ijo.IJO_3637_20 |
Open Access URL: | https://journals.lww.com/ijo/Fulltext/2022/02000/E... |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3150281 |