Stewart, Alexander, Sinclair, Emma, Ng, Joseph Chi-Fung, O'Hare, Joselli Silva, Page, Audrey, Serangeli, Ilaria, Margreitter, Christian, Orsenigo, Federica, Longman, Katherine, Frampas, Cecile et al (show 13 more authors)
(2022)
Pandemic, Epidemic, Endemic: B Cell Repertoire Analysis Reveals Unique Anti-Viral Responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus.
FRONTIERS IN IMMUNOLOGY, 13.
807104-.
Abstract
Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of <i>IGHV4-39</i> in both Zaire Ebolavirus (EBOV) and COVID-19 patient cohorts. We also show unique characteristics absent in Respiratory Syncytial Virus or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risk off-target effects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | B cell, COVID-19, ebola, RSV (respiratory syncytial virus), immunity, repertoire, class-switch recombination (CSR) |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Jun 2022 16:01 |
| Last Modified: | 18 Jan 2023 21:00 |
| DOI: | 10.3389/fimmu.2022.807104 |
| Open Access URL: | https://doi.org/10.3389/fimmu.2022.807104 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3156480 |
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