Collagen (I) homotrimer potentiates the osteogenesis imperfecta (oim) mutant allele and reduces survival in male mice

Lee, Katie J, Rambault, Lisa, Bou-Gharios, George, Clegg, Peter D ORCID: 0000-0003-0632-0032, Akhtar, Riaz ORCID: 0000-0002-7963-6874, Czanner, Gabriela, van't Hof, Rob and Canty-Laird, Elizabeth G ORCID: 0000-0001-5041-1316
(2022) Collagen (I) homotrimer potentiates the osteogenesis imperfecta (oim) mutant allele and reduces survival in male mice. DISEASE MODELS & MECHANISMS, 15 (9). dmm049428-.

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The osteogenesis imperfecta murine (oim) model with solely homotrimeric (α1)3 type I collagen, owing to a dysfunctional α2(I) collagen chain, has a brittle bone phenotype, implying that the (α1)2(α2)1 heterotrimer is required for physiological bone function. Here, we comprehensively show, for the first time, that mice lacking the α2(I) chain do not have impaired bone biomechanical or structural properties, unlike oim homozygous mice. However, Mendelian inheritance was affected in male mice of both lines, and male mice null for the α2(I) chain exhibited age-related loss of condition. Compound heterozygotes were generated to test whether gene dosage was responsible for the less-severe phenotype of oim heterozygotes, after allelic discrimination showed that the oim mutant allele was not downregulated in heterozygotes. Compound heterozygotes had impaired bone structural properties compared to those of oim heterozygotes, albeit to a lesser extent than those of oim homozygotes. Hence, the presence of heterotrimeric type I collagen in oim heterozygotes alleviates the effect of the oim mutant allele, but a genetic interaction between homotrimeric type I collagen and the oim mutant allele leads to bone fragility.

Item Type: Article
Uncontrolled Keywords: Collagen, Homotrimer, Col1a2, 2(I), Osteogenesis imperfecta, cvEDS
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 10 Oct 2022 10:02
Last Modified: 08 Feb 2023 23:56
DOI: 10.1242/dmm.049428
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