Determinants of tyrosinaemia during nitisinone therapy in alkaptonuria



Ranganath, LR, Milan, AM, Hughes, AT, Davison, AS, Khedr, M, Norman, BP, Bou-Gharios, G, Gallagher, JA, Imrich, R, Arnoux, JB
et al (show 2 more authors) (2022) Determinants of tyrosinaemia during nitisinone therapy in alkaptonuria. SCIENTIFIC REPORTS, 12 (1). 16083-.

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Abstract

Nitisinone (NIT) produces inevitable but varying degree of tyrosinaemia. However, the understanding of the dynamic adaptive relationships within the tyrosine catabolic pathway has not been investigated fully. The objective of the study was to assess the contribution of protein intake, serum NIT (sNIT) and tyrosine pathway metabolites to nitisinone-induced tyrosinaemia in alkaptonuria (AKU). Samples of serum and 24-h urine collected during SONIA 2 (Suitability Of Nitisinone In Alkaptonuria 2) at months 3 (V2), 12 (V3), 24 (V4), 36 (V5) and 48 (V6) were included in these analyses. Homogentisic acid (HGA), tyrosine (TYR), phenylalanine (PHE), hydroxyphenylpyruvate (HPPA), hydroxyphenyllactate (HPLA) and sNIT were analysed at all time-points in serum and urine. Total body water (TBW) metabolites were derived using 60% body weight. 24-h urine and TBW metabolites were summed to obtain combined values. All statistical analyses were post-hoc. 307 serum and 24-h urine sampling points were analysed. Serum TYR from V2 to V6, ranging from 478 to 1983 µmol/L were stratified (number of sampling points in brackets) into groups < 701 (47), 701-900 (105), 901-1100 (96) and > 1100 (59) µmol/L. The majority of sampling points had values greater than 900 µmol/L. sPHE increased with increasing sTYR (p < 0.001). Tyrosine, HPPA and HPLA in serum and TBW all increased with rising sTYR (p < 0.001), while HPLA/TYR ratio decreased (p < 0.0001). During NIT therapy, adaptive response to minimise TYR formation was demonstrated. Decreased conversion of HPPA to HPLA, relative to TYR, seems to be most influential in determining the degree of tyrosinaemia.

Item Type: Article
Uncontrolled Keywords: Humans, Brain Diseases, Metabolic, Inborn, Tyrosinemias, Alkaptonuria, Nitrobenzoates, Homogentisic Acid, Phenylpropionates, Cyclohexanones, Phenylalanine, Tyrosine
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 04 Oct 2022 07:00
Last Modified: 18 Jan 2023 20:41
DOI: 10.1038/s41598-022-20424-z
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3165108