Optical Tissue Clearing to Study the Intra-Pulmonary Biodistribution of Intravenously Delivered Mesenchymal Stromal Cells and Their Interactions with Host Lung Cells



Pichardo, Alejandra Hernandez, Amadeo, Francesco ORCID: 0000-0002-3868-2348, Wilm, Bettina ORCID: 0000-0002-9245-993X, Lévy, Raphaël, Ressel, Lorenzo ORCID: 0000-0002-6614-1223, Murray, Patricia and Sée, Violaine
(2022) Optical Tissue Clearing to Study the Intra-Pulmonary Biodistribution of Intravenously Delivered Mesenchymal Stromal Cells and Their Interactions with Host Lung Cells. International Journal of Molecular Sciences, 23 (22). p. 14171.

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Abstract

<jats:p>Mesenchymal stromal cells (MSCs) injected intravenously are trapped in the capillaries of the lungs and die within the first 24 h. Studying the biodistribution and fate of labelled therapeutic cells in the 3D pulmonary context is important to understand their function in this organ and gain insights into their mechanisms of action. Optical tissue clearing enables volumetric cell tracking at single-cell resolution. Thus, we compared three optical tissue-clearing protocols (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC), modified stabilised 3D imaging of solvent-cleared organs (s-DISCO) and ethyl cinnamate (ECi)) to evaluate their potential to track the biodistribution of human umbilical cord MSCs expressing the tdTomato fluorescence reporter and investigate how they interact with host cells in the mouse lung. The results showed that although CUBIC clearing is the only method that enables direct imaging of fluorescently labelled MSCs, combining s-DISCO or ECi with immunofluorescence or dye labelling allows the interaction of MSCs with endothelial and immune cells to be studied. Overall, this comparative study offers guidance on selecting an optical tissue-clearing method for cell tracking applications.</jats:p>

Item Type: Article
Uncontrolled Keywords: optical tissue clearing, mesenchymal stromal cells, mouse lung, biodistribution, cell tracking
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 24 Nov 2022 09:09
Last Modified: 18 Jan 2023 19:43
DOI: 10.3390/ijms232214171
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3166354