Circulating microparticles are increased amongst people presenting with HIV and advanced immune suppression in Malawi and correlate closely with arterial stiffness: a nested case control study



Kelly, Christine, Gurung, Rijan, Kamng'ona, Raphael, Sheha, Irene, Chammudzi, Mishek, Jambo, Kondwani ORCID: 0000-0002-3195-2210, Mallewa, Jane, Rapala, Alicja, Heyderman, Rob ORCID: 0000-0003-4573-449X, Mallon, Patrick
et al (show 3 more authors) (2022) Circulating microparticles are increased amongst people presenting with HIV and advanced immune suppression in Malawi and correlate closely with arterial stiffness: a nested case control study. Wellcome Open Res, 6. 264-.

Access the full-text of this item by clicking on the Open Access link.

Abstract

<h4>Background: </h4>  We aim to investigate whether circulating microparticle (CMPs) subsets were raised amongst people presenting with a new diagnosis of HIV and advanced immune suppression in Malawi, and whether they associated with arterial stiffness. <h4>Methods: </h4>  Microparticle characterisation and carotid femoral Pulse Wave Velocity (cfPWV) were carried out in a cohort of adults with a new HIV diagnosis and CD4 <100 cells/µL at 2 weeks post ART initiation. HIV uninfected controls were matched on age, systolic BP and diastolic BP in a 1:1 ratio.  Circulating microparticles were identified from platelet poor plasma and stained for endothelial, leucocyte, monocyte and platelet markers. <h4>Results: </h4>:  The median (IQ) total CMP count for 71 participants was 1 log higher in HIV compared to those without (p<0.0001) and was associated with arterial stiffness (spearman rho 0.47, p<0.001). In adjusted analysis, every log increase in circulating particles showed a 20% increase in cfPWV (95% CI 4 – 40%, p=0.02). In terms of subsets, endothelial and platelet derived microparticles were most strongly associated with HIV. Endothelial derived E-selectin+ CMPs were 1.3log-fold higher and platelet derived CD42a+ CMPs were 1.4log-fold higher (both p<0.0001). Endothelial and platelet derived CMPs also correlated most closely with arterial stiffness [spearman rho: E-selectin+ 0.57 and CD42a 0.56, both p<0.0001). <h4>Conclusions: </h4>:  Circulating microparticles associate strongly with arterial stiffness among PLWH in Malawi. Endothelial and platelet microparticles are the predominant cell origin types, indicating that platelet driven endothelial dysfunction pathways warrant further investigation in HIV associated arterial stiffness.

Item Type: Article
Uncontrolled Keywords: HIV, cardiovascular, microparticles
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 20 Jan 2023 09:57
Last Modified: 20 Jan 2023 09:57
DOI: 10.12688/wellcomeopenres.17044.2
Open Access URL: https://doi.org/10.12688/wellcomeopenres.17044.2
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3167183