Byrne, Dominic P, Omar, Mitchell H, Kennedy, Eileen J, Eyers, Patrick A 
ORCID: 0000-0002-9220-2966 and Scott, John D
(2022)
Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes.
Methods in molecular biology (Clifton, N.J.), 2483.
pp. 297-317.
Abstract
Generation of the prototypic second messenger cAMP instigates numerous signaling events. A major intracellular target of cAMP is Protein kinase A (PKA), a Ser/Thr protein kinase. Where and when this enzyme is activated inside the cell has profound implications on the functional impact of PKA. It is now well established that PKA signaling is focused locally into subcellular signaling "islands" or "signalosomes." The A-Kinase Anchoring Proteins (AKAPs) play a critical role in this process by dictating spatial and temporal aspects of PKA action. Genetically encoded biosensors, small molecule and peptide-based disruptors of PKA signaling are valuable tools for rigorous investigation of local PKA action at the biochemical level. This chapter focuses on approaches to evaluate PKA signaling islands, including a simple assay for monitoring the interaction of an AKAP with a tunable PKA holoenzyme. The latter approach evaluates the composition of PKA holoenzymes, in which regulatory subunits and catalytic subunits can be visualized in the presence of test compounds and small-molecule inhibitors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cyclic AMP-Dependent Protein Kinases, Peptides, Signal Transduction, Second Messenger Systems, A Kinase Anchor Proteins |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 02 Feb 2023 15:13 |
| Last Modified: | 02 Feb 2023 15:13 |
| DOI: | 10.1007/978-1-0716-2245-2_19 |
| Open Access URL: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC95186... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3168106 |
Dimensions
Dimensions
