Rapid identification of mosquito species and age by mass spectrometric analysis



Wagner, Iris ORCID: 0000-0001-9198-4532, Grigoraki, Linda, Enevoldson, Peter, Clarkson, Michael, Jones, Sam, Hurst, Jane LL ORCID: 0000-0002-3728-9624, Beynon, Robert JJ ORCID: 0000-0003-0857-495X and Ranson, Hilary
(2023) Rapid identification of mosquito species and age by mass spectrometric analysis. BMC BIOLOGY, 21 (1). 10-.

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Abstract

<h4>Background</h4>A rapid, accurate method to identify and to age-grade mosquito populations would be a major advance in predicting the risk of pathogen transmission and evaluating the public health impact of vector control interventions. Whilst other spectrometric or transcriptomic methods show promise, current approaches rely on challenging morphological techniques or simple binary classifications that cannot identify the subset of the population old enough to be infectious. In this study, the ability of rapid evaporative ionisation mass spectrometry (REIMS) to identify the species and age of mosquitoes reared in the laboratory and derived from the wild was investigated.<h4>Results</h4>The accuracy of REIMS in identifying morphologically identical species of the Anopheles gambiae complex exceeded 97% using principal component/linear discriminant analysis (PC-LDA) and 84% based on random forest analysis. Age separation into 3 different age categories (1 day, 5-6 days, 14-15 days) was achieved with 99% (PC-LDA) and 91% (random forest) accuracy. When tested on wild mosquitoes from the UK, REIMS data could determine the species and age of the specimens with accuracies of 91 and 90% respectively.<h4>Conclusions</h4>The accuracy of REIMS to resolve the species and age of Anopheles mosquitoes is comparable to that achieved by infrared spectroscopy approaches. The processing time and ease of use represent significant advantages over current, dissection-based methods. Importantly, the accuracy was maintained when using wild mosquitoes reared under differing environmental conditions, and when mosquitoes were stored frozen or desiccated. This high throughput approach thus has potential to conduct rapid, real-time monitoring of vector populations, providing entomological evidence of the impact of alternative interventions.

Item Type: Article
Uncontrolled Keywords: Rapid evaporative ionisation mass spectrometry, REIMS, Mass spectrometry, Species identification, Mosquito, Age grading
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 03 Mar 2023 15:12
Last Modified: 03 Mar 2023 15:12
DOI: 10.1186/s12915-022-01508-8
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3168742