Whole genome DNA methylation and mutational profiles identify novel changes in proliferative verrucous leukoplakia.


Okoturo, Eyituoyo, Green, Daniel, Clarke, Kim, Liloglou, Triantafyllos ORCID: 0000-0003-0460-1404, Boyd, Mark T, Shaw, Richard J ORCID: 0000-0001-7027-8997 and Risk, Janet M ORCID: 0000-0002-8770-7783 (2023) Whole genome DNA methylation and mutational profiles identify novel changes in proliferative verrucous leukoplakia. Oral surgery, oral medicine, oral pathology and oral radiology, 135 (6). pp. 893-903.

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Abstract

<h4>Objective</h4>Proliferative verrucous leukoplakia (PVL) is a rare form of oral leukoplakia with a relatively high transformation rate resulting in oral squamous cell carcinoma (OSCC). Molecular analysis of PVL at the genome level is limited and has only identified molecular similarities between PVL and OSCC. However, the clinical profile of PVL suggests that molecular differences may be more important.<h4>Study design</h4>Whole exome sequencing of 5 PVL-associated OSCC (PVL-OSCC) and paired blood samples was used to identify somatic mutations common to the tumors. Whole methylome analysis of samples from 4 PVL-associated OSCC and 3 OSCC of non-PVL origin samples was conducted to explore differential methylation.<h4>Results</h4>In contrast to conventional OSCC, PVL-associated OSCC showed infrequent TP53 mutation and altered spectra of PIK3CA and NOTCH1 mutations. Unsupervised hierarchical clustering identified 63 probes that discriminated between PVL-associated OSCC and OSCC of non-PVL origin. Differences in methylation were most significant for divalent metal ion transport, particularly calcium movement.<h4>Conclusions</h4>Specific differences in mutation and methylation profiles between PVL-derived OSCC and OSCC of non-PVL origin suggest differences in their transformation pathways. Further studies of early PVL lesions may identify markers of transformation that are also applicable to more common oral premalignant disorders such as oral epithelial dysplasia.

Item Type: Article
Uncontrolled Keywords: Humans, Carcinoma, Squamous Cell, Carcinoma, Verrucous, Head and Neck Neoplasms, Mouth Neoplasms, Leukoplakia, Oral, Cell Transformation, Neoplastic, DNA Methylation, Mutation, Squamous Cell Carcinoma of Head and Neck
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 07 Mar 2023 16:22
Last Modified: 21 Jun 2023 10:14
DOI: 10.1016/j.oooo.2023.03.004
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3168820
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