Hosmillo, Myra, Lu, Jia, McAllaster, Michael R, Eaglesham, James B, Wang, Xinjie, Emmott, Edward 
ORCID: 0000-0002-3239-8178, Domingues, Patricia, Chaudhry, Yasmin, Fitzmaurice, Tim J, Tung, Matthew KH et al (show 5 more authors)
(2019)
Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation.
ELIFE, 8.
e46681-.
Abstract
Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required for norovirus infection. The core stress granule component G3BP1 was identified as a host factor essential for efficient human and murine norovirus infection, demonstrating a conserved function across the <i>Norovirus</i> genus. Furthermore, we show that G3BP1 functions in the novel paradigm of viral VPg-dependent translation initiation, contributing to the assembly of translation complexes on the VPg-linked viral positive sense RNA genome by facilitating ribosome recruitment. Our data uncovers a novel function for G3BP1 in the life cycle of positive sense RNA viruses and identifies the first host factor with pan-norovirus pro-viral activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cell Line, Animals, Humans, Mice, Norovirus, Caliciviridae Infections, DNA Helicases, RNA Helicases, Viral Proteins, Protein Biosynthesis, Host-Pathogen Interactions, RNA Recognition Motif Proteins, Poly-ADP-Ribose Binding Proteins |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 24 Apr 2023 07:28 |
| Last Modified: | 18 Oct 2023 12:16 |
| DOI: | 10.7554/eLife.46681 |
| Open Access URL: | https://elifesciences.org/articles/46681 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3169896 |
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