Application of KU812 cells for assessing complement activation related effects by nano(bio)materials

David, Christopher ORCID: 0000-0001-8504-2354, de la Fonteyne-Blankestijn, Liset, Vermeulen, Jolanda, Plant-Hately, Alex ORCID: 0000-0003-2425-1990, Vandebriel, Robert and Liptrott, Neill ORCID: 0000-0002-5980-8966 (2023) Application of KU812 cells for assessing complement activation related effects by nano(bio)materials. Biomedicine and Pharmacotherapy, 163. 114841-.

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Abstract

Immunocompatibility issues related to nano(bio)materials, particularly liposomal formulations, involving activation of the complement system have been relatively well described however, they highlight the importance of preclinical evaluation of such interactions. These complement-mediated hypersensitivity reactions, in which basophils are implicated, are associated with complement activation-related pseudoallergy (CARPA). Ex vivo investigation of such events using primary basophils is technically challenging due to the relatively limited number of circulating basophils in peripheral blood. In the current work, the KU812 cell line has been applied as an in vitro model for basophil activation to investigate CARPA-related responses following exposure to test materials obtained from the REFINE consortium. To that end, we developed a standard operating procedure measuring a panel of cell-surface markers indicative of basophilic activation. Two laboratories performed the assays, demonstrating a clear difference in responses between liposomal and polymeric nano(bio)materials, while interlaboratory comparison of the standard operating procedure demonstrated reproducibility in results, between the two facilities. These results suggest the potential to use this protocol as a screening method for such responses however, validation using primary basophils is now warranted.

Item Type:	Article
Uncontrolled Keywords:	Basophil, Flow cytometry, Complement activation -related pseudoallergy
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	04 May 2023 09:49
Last Modified:	04 Sep 2023 12:19
DOI:	10.1016/j.biopha.2023.114841
Open Access URL:	https://doi.org/10.1016/j.biopha.2023.114841
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3170139