Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera.



Roper, Kelly J ORCID: 0000-0002-4616-4313, Thomas, Jordan, Albalawi, Wejdan, Maddocks, Emily, Dobson, Susan, Alshehri, Abdullateef, Barone, Francesco G, Baltazar, Murielle ORCID: 0000-0002-1972-2308, Semple, Malcolm G ORCID: 0000-0001-9700-0418, Ho, Antonia
et al (show 4 more authors) (2023) Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera. Scientific reports, 13 (1). 15014-.

[thumbnail of Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera.pdf] PDF
Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera.pdf - Open Access published version

Download (1MB) | Preview

Abstract

The ongoing SARS-CoV-2 pandemic was initially managed by non-pharmaceutical interventions such as diagnostic testing, isolation of positive cases, physical distancing and lockdowns. The advent of vaccines has provided crucial protection against SARS-CoV-2. Neutralising antibody (nAb) responses are a key correlate of protection, and therefore measuring nAb responses is essential for monitoring vaccine efficacy. Fingerstick dried blood spots (DBS) are ideal for use in large-scale sero-surveillance because they are inexpensive, offer the option of self-collection and can be transported and stored at ambient temperatures. Such advantages also make DBS appealing to use in resource-limited settings and in potential future pandemics. In this study, nAb responses in sera, venous blood and fingerstick blood stored on filter paper were measured. Samples were collected from SARS-CoV-2 acutely infected individuals, SARS-CoV-2 convalescent individuals and SARS-CoV-2 vaccinated individuals. Good agreement was observed between the nAb responses measured in eluted DBS and paired sera. Stability of nAb responses was also observed in sera stored on filter paper at room temperature for 28 days. Overall, this study provides support for the use of filter paper as a viable sample collection method to study nAb responses.

Item Type: Article
Uncontrolled Keywords: ISARIC4C Consortium, Humans, Communicable Disease Control, Biological Transport, Antibodies, Neutralizing, COVID-19, SARS-CoV-2
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Faculty of Health and Life Sciences > Institute of Population Health
Faculty of Health and Life Sciences > Clinical Directorate
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 22 Sep 2023 15:23
Last Modified: 29 Sep 2023 09:32
DOI: 10.1038/s41598-023-41928-2
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3172978