Sun, Yaqi, Harman, Victoria, Johnson, James, Chen, Taiyu, Dykes, Gregory, Lin, Yongjun, Beynon, Robert and Liu, Lu-Ning ORCID: 0000-0002-8884-4819
(2021)
Decoding the absolute stoichiometric composition and structural plasticity of α-carboxysomes.
bioRxiv.
2021.12.06.471529-2021.12.06.471529.
Text
2021_11 a-CB QconCAT.pdf - Submitted version Download (6MB) | Preview |
Abstract
Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria and some chemoautotrophs. This self-assembling organelle encapsulates the key CO 2 -fixing enzymes, Rubisco, and carbonic anhydrase using a polyhedral protein shell that is constructed by hundreds of shell protein paralogs. The α-carboxysome from the chemoautotroph Halothiobacillus neapolitanus serves as a model system in fundamental studies and synthetic engineering of carboxysomes. Here we adopt a QconCAT-based quantitative mass spectrometry to determine the absolute stoichiometric composition of native α-carboxysomes from H. neapolitanus . We further performed an in-depth comparison of the protein stoichiometry of native and recombinant α-carboxysomes heterologously generated in Escherichia coli to evaluate the structural variability and remodeling of α-carboxysomes. Our results provide insight into the molecular principles that mediate carboxysome assembly, which may aid in rational design and reprogramming of carboxysomes in new contexts for biotechnological applications.
Item Type: | Article |
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Uncontrolled Keywords: | Biotechnology |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
Depositing User: | Symplectic Admin |
Date Deposited: | 14 Dec 2021 10:25 |
Last Modified: | 15 Mar 2024 12:09 |
DOI: | 10.1101/2021.12.06.471529 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3145332 |