SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.

Sadler, Joanna C, Swainston, Neil, Dunstan, Mark S, Currin, Andrew and Kell, Douglas B ORCID: 0000-0001-5838-7963 (2022) SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution. Methods in molecular biology (Clifton, N.J.), 2461. pp. 67-83.

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Official URL: http://dx.doi.org/10.1007/978-1-0716-2152-3_5

Abstract

Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as k<sub>cat,</sub> thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein's vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.

Item Type:	Article
Uncontrolled Keywords:	Proteins, Directed Molecular Evolution, Gene Library
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	20 Oct 2022 08:48
Last Modified:	22 Jun 2023 01:30
DOI:	10.1007/978-1-0716-2152-3_5
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3165539