Sadler, Joanna C, Swainston, Neil, Dunstan, Mark S, Currin, Andrew and Kell, Douglas B ORCID: 0000-0001-5838-7963
(2022)
SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.
Methods in molecular biology (Clifton, N.J.), 2461.
pp. 67-83.
Text
Speedy Genes XL Sadler et al AAM .docx - Author Accepted Manuscript Download (248kB) |
Abstract
Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as k<sub>cat,</sub> thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein's vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.
Item Type: | Article |
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Uncontrolled Keywords: | Proteins, Directed Molecular Evolution, Gene Library |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
Depositing User: | Symplectic Admin |
Date Deposited: | 20 Oct 2022 08:48 |
Last Modified: | 22 Jun 2023 01:30 |
DOI: | 10.1007/978-1-0716-2152-3_5 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3165539 |