Azzariti, Stefano, Bond, Ross, Loeffler, Anette, Zendri, Flavia, Timofte, Dorina 
ORCID: 0000-0002-7261-738X, Chang, Yu-Mei and Pelligand, Ludovic
(2022)
Investigation of In Vitro Susceptibility and Resistance Mechanisms in Skin Pathogens: Perspectives for Fluoroquinolone Therapy in Canine Pyoderma.
ANTIBIOTICS-BASEL, 11 (9).
1204-.
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Investigation of In Vitro Susceptibility and Resistance Mechanisms in Skin Pathogens Perspectives for Fluoroquinolone Therap.pdf - Published version Download (662kB) | Preview |
Abstract
Fluoroquinolones (FQ) are commonly used in dogs with bacterial skin infections. Their use as first choice, along with the increased incidence of FQ-resistance, represents a risk to animal and public health. Our study determined minimum inhibitory (MIC) and bactericidal (MBC) concentrations of five FQs in <i>Staphylococcus aureus</i>, <i>Staphylococcus pseudintermedius,</i> and <i>Escherichia coli,</i> together with FQ-resistance mechanisms. MICs, efflux pump (EP) overexpression and MBCs were measured in 249 skin infection isolates following CLSI guidelines (CLSI VET01-A4, CLSI M26-A). Chromosomal and plasmid-mediated resistance genes were investigated after DNA extraction and sequencing. FQ-resistance was detected in 10% of methicillin-susceptible (MS), 90% of methicillin-resistant (MR) staphylococci and in 36% of <i>E. coli</i>. Bactericidal effect was observed except in 50% of MRSA/P for ciprofloxacin and in 20% of MRSPs for enrofloxacin. Highest MICs were associated with double mutation in <i>gyrA</i> (Ser83Leu + Asp87Asn), efflux pumps and three PMQR genes in <i>E. coli</i>, and <i>grlA</i> (Ser80Phe + Glu84Lys) in <i>S. aureus</i>. EP overexpression was high among <i>E. coli</i> (96%), low in <i>S. aureus</i> (1%) and absent in <i>S. pseudintermedius</i>. Pradofloxacin and moxifloxacin showed low MICs with bactericidal effect. Since in vitro FQ resistance was associated with MR, FQ use should be prudently guided by susceptibility testing.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | canine pyoderma, staphylococci, S, pseudintermedius, S, aureus, E, coli, antimicrobial resistance, methicillin-resistance |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 31 Jan 2023 12:49 |
| Last Modified: | 31 Jan 2023 12:49 |
| DOI: | 10.3390/antibiotics11091204 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3168021 |
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