Kepczynska, Malgorzata A, Zaibi, Mohamed S, Alomar, Suliman Y and Trayhurn, Paul 
ORCID: 0000-0002-9940-283X
(2017)
PCR arrays indicate that the expression of extracellular matrix and cell adhesion genes in human adipocytes is regulated by IL-1 (interleukin-1).
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, 123 (1).
pp. 61-67.
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Abstract
The role of IL-1β in regulating the expression of extracellular matrix (ECM) and cell adhesion genes in human adipocytes has been examined. Adipocytes differentiated in culture were incubated with IL-1β for 4 or 24 h and RNA probed with PCR arrays for 84 ECM and cell adhesion genes. Treatment with IL-1β resulted in changes in the expression at one or both time points of ∼50% of the genes probed by the arrays, the majority being down-regulated. Genes whose expression was down-regulated by IL-1β included those encoding several collagen chains and integrin subunits. In contrast, IL-1β induced substantial increases (>10-fold) in the expression of ICAM1, VCAM1, MMP1 and MMP3; the secretion of the encoded proteins was also markedly stimulated. IL-1β has a pervasive effect on the expression of ECM and cell adhesion genes in human adipocytes, consistent with the derangement of tissue structure during inflammation in white fat.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Adipose tissue, fibrosis, ICAM1, MMPs, VCAM1 |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 10 Jul 2023 13:15 |
| Last Modified: | 10 Jul 2023 13:15 |
| DOI: | 10.1080/13813455.2016.1248979 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3171609 |
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