Sohn, Y-S, Shin, H-C, Park, WS, Ge, J, Kim, CH, Bok, LL, Heo, WD, Jung, JU, Rigden, D ORCID: 0000-0002-7565-8937 and Oh, BH
(2015)
Lpg0393 of Legionella pneumophila is a guanine-nucleotide exchange factor for Rab5, Rab21 and Rab22.
PLoS ONE, 10 (3).
e0118683-.
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Abstract
Legionella pneumophila, a human intracellular pathogen, encodes about 290 effector proteins that are translocated into host cells through a secretion machinery. Some of these proteins have been shown to manipulate or subvert cellular processes during infection, but functional roles of a majority of them remain unknown. Lpg0393 is a newly identified Legionella effector classified as a hypothetical protein. Through X-ray crystallographic analysis, we show that Lpg0393 contains a Vps9-like domain, which is structurally most similar to the catalytic core of human Rabex-5 that activates the endosomal Rab proteins Rab5, Rab21 and Rab22. Consistently, Lpg0393 exhibited a guanine-nucleotide exchange factor activity toward the endosomal Rabs. This work identifies the first example of a bacterial guanine-nucleotide exchange factor that is active towards the Rab5 sub-cluster members, implying that the activation of these Rab proteins might be advantageous for the intracellular survival of Legionella.
Item Type: | Article |
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Uncontrolled Keywords: | Golgi Apparatus, Humans, Legionella pneumophila, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, Guanine Nucleotide Exchange Factors, Bacterial Proteins, Sequence Alignment, Amino Acid Sequence, Protein Conformation, Protein Binding, Protein Transport, Models, Molecular, Molecular Sequence Data, Protein Interaction Domains and Motifs |
Depositing User: | Symplectic Admin |
Date Deposited: | 29 Jan 2015 09:55 |
Last Modified: | 15 Dec 2022 12:18 |
DOI: | 10.1371/journal.pone.0118683 |
Open Access URL: | https://journals.plos.org/plosone/article?id=10.13... |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/2006201 |