Lpg0393 of Legionella pneumophila is a guanine-nucleotide exchange factor for Rab5, Rab21 and Rab22


Sohn, Y-S, Shin, H-C, Park, WS, Ge, J, Kim, CH, Bok, LL, Heo, WD, Jung, JU, Rigden, D ORCID: 0000-0002-7565-8937 and Oh, BH (2015) Lpg0393 of Legionella pneumophila is a guanine-nucleotide exchange factor for Rab5, Rab21 and Rab22. PLoS ONE, 10 (3). e0118683-.

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Abstract

Legionella pneumophila, a human intracellular pathogen, encodes about 290 effector proteins that are translocated into host cells through a secretion machinery. Some of these proteins have been shown to manipulate or subvert cellular processes during infection, but functional roles of a majority of them remain unknown. Lpg0393 is a newly identified Legionella effector classified as a hypothetical protein. Through X-ray crystallographic analysis, we show that Lpg0393 contains a Vps9-like domain, which is structurally most similar to the catalytic core of human Rabex-5 that activates the endosomal Rab proteins Rab5, Rab21 and Rab22. Consistently, Lpg0393 exhibited a guanine-nucleotide exchange factor activity toward the endosomal Rabs. This work identifies the first example of a bacterial guanine-nucleotide exchange factor that is active towards the Rab5 sub-cluster members, implying that the activation of these Rab proteins might be advantageous for the intracellular survival of Legionella.

Item Type: Article
Uncontrolled Keywords: Golgi Apparatus, Humans, Legionella pneumophila, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, Guanine Nucleotide Exchange Factors, Bacterial Proteins, Sequence Alignment, Amino Acid Sequence, Protein Conformation, Protein Binding, Protein Transport, Models, Molecular, Molecular Sequence Data, Protein Interaction Domains and Motifs
Depositing User: Symplectic Admin
Date Deposited: 29 Jan 2015 09:55
Last Modified: 15 Dec 2022 12:18
DOI: 10.1371/journal.pone.0118683
Open Access URL: https://journals.plos.org/plosone/article?id=10.13...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/2006201
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