A quantitative and temporal map of proteostasis during heat shock in <i>Saccharomyces cerevisiae</i>



Jarnuczak, Andrew F, Albornoz, Manuel Garcia, Eyers, Claire E ORCID: 0000-0002-3223-5926, Grant, Christopher M and Hubbard, Simon J
(2018) A quantitative and temporal map of proteostasis during heat shock in <i>Saccharomyces cerevisiae</i>. MOLECULAR OMICS, 14 (1). pp. 37-52.

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Abstract

Temperature fluctuation is a common environmental stress that elicits a molecular response in order to maintain intracellular protein levels. Here, for the first time, we report a comprehensive temporal and quantitative study of the proteome during a 240 minute heat stress, using label-free mass spectrometry. We report temporal expression changes of the hallmark heat stress proteins, including many molecular chaperones, tightly coupled to their protein clients. A notable lag of 30 to 120 minutes was evident between transcriptome and proteome levels for differentially expressed genes. This targeted molecular response buffers the global proteome; fewer than 15% of proteins display significant abundance change. Additionally, a parallel study in a Hsp70 chaperone mutant (ssb1Δ) demonstrated a significantly attenuated response, at odds with the modest phenotypic effects that are observed on growth rate. We cast the global changes in temporal protein expression into protein interaction and functional networks, to afford a unique, time-resolved and quantitative description of the heat shock response in an important model organism.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 09 Jan 2018 14:03
Last Modified: 14 Oct 2023 08:42
DOI: 10.1039/c7mo00050b
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3015718