TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway

D'Ignazio, Laura, Batie, Michael ORCID: 0000-0002-7508-6641 and Rocha, Sonia (2018) TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway. CELLS, 7 (8). E102-.

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Abstract

Non-canonical NF-κB signalling plays important roles in the development and function of the immune system but it also is deregulated in a number of inflammatory diseases. Although, NF-κB and HIF crosstalk has been documented, this has only been described following canonical NF-κB stimulation, involving RelA/p50 and the HIF-1 dimer. Here, we report that the non-canonical inducer TNFSF14/LIGHT leads to HIF induction and activation in cancer cells. We demonstrate that only HIF-2α is induced at the transcriptional level following non-canonical NF-κB activation, via a mechanism that is dependent on the p52 subunit. Furthermore, we demonstrate that p52 can bind to the HIF-2α promoter in cells. These results indicate that non-canonical NF-κB can lead to HIF signalling implicating HIF-2α as one of the downstream effectors of this pathway in cells.

Item Type:	Article
Uncontrolled Keywords:	HIF, NF-kappa B, EPAS1, LIGHT, TNFSF14, p52, NIK, ChIP
Depositing User:	Symplectic Admin
Date Deposited:	04 Oct 2018 08:21
Last Modified:	17 Nov 2023 04:46
DOI:	10.3390/cells7080102
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3027088