D'Ignazio, Laura, Batie, Michael ORCID: 0000-0002-7508-6641 and Rocha, Sonia
(2018)
TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway.
CELLS, 7 (8).
E102-.
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TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway.pdf - Published version Download (2MB) |
Abstract
Non-canonical NF-κB signalling plays important roles in the development and function of the immune system but it also is deregulated in a number of inflammatory diseases. Although, NF-κB and HIF crosstalk has been documented, this has only been described following canonical NF-κB stimulation, involving RelA/p50 and the HIF-1 dimer. Here, we report that the non-canonical inducer TNFSF14/LIGHT leads to HIF induction and activation in cancer cells. We demonstrate that only HIF-2α is induced at the transcriptional level following non-canonical NF-κB activation, via a mechanism that is dependent on the p52 subunit. Furthermore, we demonstrate that p52 can bind to the HIF-2α promoter in cells. These results indicate that non-canonical NF-κB can lead to HIF signalling implicating HIF-2α as one of the downstream effectors of this pathway in cells.
Item Type: | Article |
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Uncontrolled Keywords: | HIF, NF-kappa B, EPAS1, LIGHT, TNFSF14, p52, NIK, ChIP |
Depositing User: | Symplectic Admin |
Date Deposited: | 04 Oct 2018 08:21 |
Last Modified: | 17 Nov 2023 04:46 |
DOI: | 10.3390/cells7080102 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3027088 |