Poly-ε-Lysine or Mel4 Antimicrobial Surface Modification on a Novel Peptide Hydrogel Bandage Contact Lens

Lace, Rebecca, Doherty, Kyle ORCID: 0000-0002-7616-1295, Dutta, Debarun, Willcox, Mark and Williams, Rachel ORCID: 0000-0002-1954-0256
(2020) Poly-ε-Lysine or Mel4 Antimicrobial Surface Modification on a Novel Peptide Hydrogel Bandage Contact Lens. Advanced Materials Interfaces.

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Microbial keratitis (MK) is a serious issue in many countries and is often caused by contact lens wear. Antimicrobial peptides (AMPs) are a potentially useful tool for creating antimicrobial surfaces in light of increasing antibiotic resistance. Poly--lysine (pK) is an AMP that has been used extensively as a food preservative and Mel4 has recently been synthesised and studied as an antimicrobial coating for contact lenses. A hydrogel synthesised of pK cross-linked with biscarboxylic acids provides a potential lens material which has many surface free amines, that can be subsequently used to attached additional AMPs, creating an antimicrobial lens. The aim of this study was to investigate pεK hydrogels against a clinical strain of Pseudomonas aeruginosa for preventing or treating MK. Covalent attachment of AMPs was investigated and confirmed by fluorescently tagged peptides. Bound pK effectively reduced the number of adherent P. aeruginosa in vitro (>3 log). In ex vivo studies positive antimicrobial activity was observed on bare pK hydrogels and those with additionally bound pK or Mel4; lenses allowed the maintenance of the corneal epithelium. A pεK hydrogel contact lens with additional AMPs could be a therapeutic tool to reduce the incidence of MK.

Item Type: Article
Uncontrolled Keywords: antimicrobial peptides, contact lens, microbial keratitis, Pseudonomas
Depositing User: Symplectic Admin
Date Deposited: 22 Sep 2020 09:37
Last Modified: 03 Sep 2022 07:14
DOI: 10.1002/admi.202001232
Open Access URL: https://doi.org/10.1002/admi.202001232
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3102161

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