Assessment of changes in autophagic vesicles in human immune cell lines exposed to nano particles

David, Christopher AW ORCID: 0000-0001-8504-2354, del Castillo Busto, M Estela, Cuello-Nunez, Susana, Goenaga-Infante, Heidi, Barrow, Michael, Fernig, David G ORCID: 0000-0003-4875-4293, Murray, Patricia, Rosseinsky, Matthew J ORCID: 0000-0002-1910-2483, Owen, Andrew ORCID: 0000-0002-9819-7651 and Liptrott, Neill J ORCID: 0000-0002-5980-8966
(2021) Assessment of changes in autophagic vesicles in human immune cell lines exposed to nano particles. CELL AND BIOSCIENCE, 11 (1). 133-.

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Assessment of, human, immune cell lines as models for determining the impact of nanomaterials on autophagy-NJL.docx - Author Accepted Manuscript

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<h4>Background</h4>Safe and rational development of nanomaterials for clinical translation requires the assessment of potential biocompatibility. Autophagy, a critical homeostatic pathway intrinsically linked to cellular health and inflammation, has been shown to be affected by nanomaterials. It is, therefore, important to be able to assess possible interactions of nanomaterials with autophagic processes.<h4>Results</h4>CEM (T cell), Raji (B lymphocyte), and THP-1 (human monocyte) cell lines were subject to treatment with rapamycin and chloroquine, known to affect the autophagic process, in order to evaluate cell line-specific responses. Flow cytometric quantification of a fluorescent autophagic vacuole stain showed that maximum observable effects (105%, 446%, and 149% of negative controls) were achieved at different exposure durations (8, 6, and 24 h for CEM, Raji, and THP-1, respectively). THP-1 was subsequently utilised as a model to assess the autophagic impact of a small library of nanomaterials. Association was observed between hydrodynamic size and autophagic impact (r<sup>2</sup> = 0.11, p = 0.004). An ELISA for p62 confirmed the greatest impact by 10 nm silver nanoparticles, abolishing p62, with 50 nm silica and 180 nm polystyrene also lowering p62 to a significant degree (50%, 74%, and 55%, respectively, p < 0.05).<h4>Conclusions</h4>This data further supports the potential for a variety of nanomaterials to interfere with autophagic processes which, in turn, may result in altered cellular function and viability. The association of particle size with impact on autophagy now warrants further investigation.

Item Type: Article
Uncontrolled Keywords: Autophagy, Nanomaterials, Nanotoxicology
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 08 Jul 2021 08:18
Last Modified: 18 Jan 2023 21:36
DOI: 10.1186/s13578-021-00648-8
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