A Validated Method for Quantification of Efavirenz in Dried Blood Spots Using High-Performance Liquid Chromatography-Mass Spectrometry

Amara, Alieu B ORCID: 0000-0002-1137-2948, Else, Laura J, Tjia, John, Olagunju, Adeniyi ORCID: 0000-0002-6588-5749, Puls, Rebekah L, Khoo, Saye ORCID: 0000-0002-2769-0967 and Back, David J (2015) A Validated Method for Quantification of Efavirenz in Dried Blood Spots Using High-Performance Liquid Chromatography-Mass Spectrometry. THERAPEUTIC DRUG MONITORING, 37 (2). pp. 220-228.

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Abstract

<h4>Background</h4>Efavirenz (EFV) is one of the preferred components of first-line antiretroviral treatment. EFV is characterized by a long plasma half-life (40-55 hours) with large interpatient variability, which raises the potential for individualization of therapy. Analyses of EFV levels in plasma require specialized facilities (cold storage/transport) which, in resource-limited settings, can be problematic; dried blood spots (DBS)-EFV measurements thus provide a cheap easy alternative for therapeutic drug monitoring. Our aim was to develop and validate a liquid chromatography-mass spectrometry method to quantify EFV in DBS collected as part of clinical trials in resource-limited settings.<h4>Methods</h4>DBS for standards, quality control samples, and patient samples were excised and then extracted with ethyl acetate/n-hexane (50/50 vol/vol) after addition of internal standard hexobarbital, and 1 mol/L K2CO3. The extract was evaporated to dryness, the residue reconstituted in mobile phase and analyzed directly by liquid chromatography-mass spectrometry. Gradient elution was on a reverse-phase C18 column using 1 mmol/L ammonium acetate in water and acetonitrile. Quantification was by selected reaction monitoring in negative ionization mode. DBS samples were obtained at several time points over 24 hours from HIV+ patients on either 400 or 600 mg EFV in combination with emtricitabine/tenofovir.<h4>Results</h4>The internal standard and EFV eluted at 2.68 and 3.54 minutes, respectively in a 5-minute run time. Matrix effects were minimal (-5.4%). Calibration curves were validated over a concentration range of 25-5000 ng/mL. Intra-assay and interassay variations ranged between 6.7% and 8.7% for imprecision and 100.3% and 104.2% for accuracy. Mean recovery was >64%. The DBS data showed a strong positive correlation with a validated plasma EFV assay (R = 0.9764, P < 0.001). EFV concentrations from DBS were approximately 42% lower than the paired plasma values, and the ratio of blood/plasma did not change over the dosing interval.<h4>Conclusions</h4>The validated assay is now routinely applied to clinical samples measuring DBS EFV for pharmacokinetic analysis. The methodology is robust, accurate, and sensitive.

Item Type:	Article
Additional Information:	Amara, Alieu B Else, Laura J Tjia, John Olagunju, Adeniyi Puls, Rebekah L Khoo, Saye Back, David J ENG 2014/08/28 06:00 Ther Drug Monit. 2014 Aug 26.## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	efavirenz, pharmacokinetics, dried blood spots, blood/plasma ratio, liquid chromatography-mass spectrometry
Depositing User:	Symplectic Admin
Date Deposited:	03 Jun 2015 10:56
Last Modified:	15 Dec 2022 15:29
DOI:	10.1097/FTD.0000000000000127
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2011760