Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue‐engineered skin equivalents

Smith, Sarah, Colley, Helen, Sharma, P ORCID: 0000-0002-5534-2417, Slowik, Klaudia, Sison-Young, Rowena, Sneddon, Andrew, Webb, Steven and Murdoch, Craig (2018) Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue‐engineered skin equivalents. Experimental Dermatology, 27 (5). pp. 473-475.

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Official URL: https://onlinelibrary.wiley.com/doi/full/10.1111/e...

Abstract

CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue‐engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl‐O‐methyl‐cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time‐dependent increase in metabolite production, confirming the functional expression of these enzymes in skin.

Item Type:	Article
Uncontrolled Keywords:	epithelium, steroids, three-dimensional tissue models, toxicity, xenobiotic metabolism
Depositing User:	Symplectic Admin
Date Deposited:	14 Dec 2017 09:34
Last Modified:	19 Jan 2023 06:48
DOI:	10.1111/exd.13483
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3014132