Sayin, Esen, Rashid, Rosti Hama, Carlos Rodriguez-Cabello, Jose, Elsheikh, Ahmed 
ORCID: 0000-0001-7456-1749, Baran, Erkan Turker and Hasirci, Vasif
(2017)
Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend.
BIOACTIVE MATERIALS, 2 (2).
pp. 71-81.
Abstract
The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen - silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimate tensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28 days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of 0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young's modulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better than the HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissue engineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown the enhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by using ADSCs.
| Item Type: | Article |
|---|---|
| Additional Information: | publisher: Elsevier articletitle: Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend journaltitle: Bioactive Materials articlelink: http://dx.doi.org/10.1016/j.bioactmat.2017.04.001 content_type: article copyright: © 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. |
| Uncontrolled Keywords: | Adipose-derived stem cells, Human osteoblasts, Tissue engineering, Bone, Mechanical properties |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 23 Jan 2018 07:31 |
| Last Modified: | 19 Jan 2023 06:42 |
| DOI: | 10.1016/j.bioactmat.2017.04.001 |
| Open Access URL: | https://www.sciencedirect.com/science/article/pii/... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3016658 |
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