Keratocyte Density Is Reduced and Related to Corneal Nerve Damage in Diabetic Neuropathy.

Kalteniece, Alise ORCID: 0000-0002-0180-513X, Ferdousi, Maryam ORCID: 0000-0002-7989-8233, Azmi, Shazli, Marshall, Andrew ORCID: 0000-0001-8273-7089, Soran, Handrean and Malik, Rayaz A (2018) Keratocyte Density Is Reduced and Related to Corneal Nerve Damage in Diabetic Neuropathy. Investigative ophthalmology & visual science, 59 (8). pp. 3584-3590.

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Official URL: http://dx.doi.org/10.1167/iovs.18-23889

Abstract

<h4>Purpose</h4>The purpose of this study was to assess the relationship between corneal keratocyte density (KD) and corneal nerve damage in patients with and without diabetic peripheral neuropathy.<h4>Methods</h4>Eighty-six patients with type 1 and type 2 diabetes and 21 age-matched control subjects underwent assessment of the neuropathy disability score, quantitative sensory testing, electrophysiology, and corneal confocal microscopy and were divided into those without (DN-) (n = 22) and with (DN+) (n = 64) diabetic neuropathy. Corneal sub-basal nerve parameters and KD in the anterior, mid, and posterior stroma were quantified.<h4>Results</h4>Anterior, mid, and posterior stromal KD were significantly reduced in DN- (P = 0.02, P = 0.009, P = 0.01, respectively) and DN+ (all P < 0.0001) subjects compared to controls. Corneal nerve branch density (CNBD) (P < 0.0001, P < 0.0001) and corneal nerve fiber length (CNFL) (P = 0.03, P < 0.0001) were significantly reduced in DN- and DN+ subjects, respectively, and corneal nerve fiber density (CNFD) (P < 0.0001) was significantly reduced only in DN+ subjects compared to controls. Anterior, mid, and posterior stromal KD correlated significantly with CNFD (P = 0.008, P = 0.005, P = 0.01), CNBD (P = 0.01, P = 0.006, P = 0.001), and CNFL (P = 0.04, P = 0.008, P = 0.003), respectively.<h4>Conclusions</h4>This study demonstrates a reduction in anterior, mid, and posterior KD, which is associated with corneal sub-basal plexus nerve damage in patients with diabetes.

Item Type:	Article
Uncontrolled Keywords:	Nerve Tissue, Nerve Fibers, Cornea, Humans, Diabetic Neuropathies, Diabetes Mellitus, Type 1, Diabetes Mellitus, Type 2, Microscopy, Confocal, Cell Count, ROC Curve, Middle Aged, Female, Male, Corneal Keratocytes
Depositing User:	Symplectic Admin
Date Deposited:	12 Aug 2019 10:50
Last Modified:	15 Mar 2024 19:36
DOI:	10.1167/iovs.18-23889
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3051348