Insights Into Peptide Inhibition of Alpha-Synuclein Aggregation

Torpey, James H, Meade, Richard M, Mistry, Ravina, Mason, Jody M and Madine, Jillian ORCID: 0000-0001-9963-5871 (2020) Insights Into Peptide Inhibition of Alpha-Synuclein Aggregation. FRONTIERS IN NEUROSCIENCE, 14. 561462-.

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Official URL: http://dx.doi.org/10.3389/fnins.2020.561462

Abstract

α-Synuclein (aSyn) aggregation is an attractive target for therapeutic development for a range of neurodegenerative conditions, collectively termed synucleinopathies. Here, we probe the mechanism of action of a peptide 4554W, (KDGIVNGVKA), previously identified through intracellular library screening, to prevent aSyn aggregation and associated toxicity. We utilize NMR to probe association and identify that 4554W associates with a "partially aggregated" form of aSyn, with enhanced association occurring over time. We also report the ability of 4554W to undergo modification through deamidation of the central asparagine residue, occurring on the same timescale as aSyn aggregation <i>in vitro</i>, with peptide modification enhancing its association with aSyn. Additionally, we report that 4554W can act to reduce fibril formation of five Parkinson's disease associated aSyn mutants. Inhibitory peptide binding to partially aggregated forms of aSyn, as identified here, is particularly attractive from a therapeutic perspective, as it would eliminate the need to administer the therapy at pre-aggregation stages, which are difficult to diagnose. Taken together the data suggest that 4554W could be a suitable candidate for future therapeutic development against wild-type, and most mutant aSyn aggregation.

Item Type:	Article
Uncontrolled Keywords:	alpha-synuclein, electron microscopy, neurodegenerative disease, NMR, Parkinson disease, peptide interaction, protein aggregation
Depositing User:	Symplectic Admin
Date Deposited:	04 Nov 2020 15:06
Last Modified:	18 Jan 2023 23:23
DOI:	10.3389/fnins.2020.561462
Open Access URL:	https://doi.org/10.3389/fnins.2020.561462
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3106044