Non-nutritive sweetener activation of the pig sweet taste receptor T1R2-T1R3 in vitro mirrors sweetener stimulation of the gut- expressed receptor in vivo

Daly, Kristian ORCID: 0000-0002-1075-1831, Al-Rammahi, Miran ORCID: 0000-0002-0165-7990, Moran, Andrew W ORCID: 0000-0003-0031-2154, Weatherburn, Darren and Shirazi-Beechey, Soraya (2021) Non-nutritive sweetener activation of the pig sweet taste receptor T1R2-T1R3 in vitro mirrors sweetener stimulation of the gut- expressed receptor in vivo. Biochemical and Biophysical Research Communications, 542. pp. 54-58.

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2021.01.032

Abstract

The perception of sweet is mediated by the sweet taste receptor T1R2-T1R3 expressed in taste cells of the lingual epithelium. This receptor is also expressed in intestinal enteroendocrine cells and is required for sensing luminal sugars and sweeteners to regulate expression of intestinal Na<sup>+</sup>-glucose cotransporter 1 (SGLT1). There are some notable differences amongst species in the ability to detect certain non-nutritive (artificial) sweeteners. Amino acid substitutions and pseudogenization of taste receptor genes are responsible for these disparities. Using heterologous expression, we demonstrate that the commonly used non-nutritive sweeteners sucralose, saccharin and acesulfame K activate pig T1R2-T1R3, but that aspartame and cyclamate do not. Furthermore, we show that in vitro sweetener activation of pig T1R2-T1R3 mirrors the sweetener stimulation of the gut-expressed receptor in vivo. Considering that sweeteners are included in animal feed worldwide, determination of taste receptor specificities in different species is essential for the development of scientifically-based dietary formulations.

Item Type:	Article
Uncontrolled Keywords:	Taste, T1R2-T1R3, Non-nutritive sweeteners, Pig, Intestine
Depositing User:	Symplectic Admin
Date Deposited:	13 Jan 2021 09:41
Last Modified:	17 Apr 2023 13:10
DOI:	10.1016/j.bbrc.2021.01.032
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3113395