Nadal-Jimenez, Pol 
ORCID: 0000-0002-9883-0920, Siozios, Stefanos ORCID: 0000-0002-1104-7061, Halliday, Nigel, Camara, Miguel and Hurst, Gregory DD ORCID: 0000-0002-7163-7784
(2022)
<i>Symbiopectobacterium purcellii</i>, gen. nov., sp. nov., isolated from the leafhopper <i>Empoasca decipiens</i>.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 72 (6).
Abstract
Bacterial endosymbionts are found in multiple arthropod species, where they play crucial roles as nutritional symbionts, defensive symbionts or reproductive parasites. Recent work has highlighted a new clade of heritable microbes within the gammaproteobacteria that enter into both obligate and facultative symbioses, with an obligately required unculturable symbiont recently given the name <i>Candidatus</i> Symbiopectobacterium. In this study, we describe a culturable rod shaped non-flagellated bacterial symbiont from this clade isolated from the leafhopper <i>Empoasca decipiens</i>. The symbiont is related to the transovarially transmitted 'BEV' bacterium that was first isolated from the leafhopper <i>Euscelidius variegatus</i> by Alexander Purcell, and we therefore name the symbiont <i>Symbiopectobacterium purcellii</i> sp. nov., gen. nov. We further report the closed genome sequence for <i>S. purcellii</i>. The genome is atypical for a heritable microbe, being large in size, without profound AT bias and with little evidence of pseudogenization. The genome is predicted to encode Type II, III and VI secretion systems and associated effectors and a non-ribosomal peptide synthase array likely to produce bioactive small molecules. The predicted metabolism is more complete than for other symbionts in the <i>Symbiopectobacterium</i> clade, and the microbe is predicted to synthesize a range of B vitamins. However, Biolog plate results indicate that the metabolism is depauperate compared to the sister clade, represented by <i>Pectobacterium carotovorum</i>. A quorum-sensing pathway related to that of <i>Pectobacterium</i> species (containing an overlapping expI-expR1 pair in opposite directions and a "solo" expR2) is evidenced, and LC-MS/MS analysis reveals the presence of 3-hydroxy-C10-HSL as the sole <i>N</i>-acylhomoserine lactone (AHL) in our strain. This AHL profile is profoundly divergent from that of other <i>Erwinia</i> and <i>Pectobacterium</i> species which produce mostly 3-oxo-C6- and 3-oxo-C8-HSL and could aid group identification. Thus, this microbe denotes one that has lost certain pathways associated with a saprophytic lifestyle but represents an important baseline against which to compare other members of the genus <i>Symbiopectobacterium</i> that show more profound integration into host biology. The type strain of <i>Symbiopectobacterium purcellii</i> gen. nov., sp. nov. is SyEd1<sup>T</sup> (LMG 32449<sup>T</sup>=CECT 30436<sup>T</sup>).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Empoasca, leafhopper, quorum sensing, symbiosis, Symbiopectobacterium |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 22 Jun 2022 09:19 |
| Last Modified: | 17 Oct 2023 16:27 |
| DOI: | 10.1099/ijsem.0.005440 |
| Open Access URL: | https://www.microbiologyresearch.org/content/journ... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3156923 |
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