Lysosome exocytosis is required for mitosis in mammalian cells

Nugues, Charlotte, Rajamanoharan, Dayani, Burgoyne, Robert D ORCID: 0000-0002-9219-0387, Haynes, Lee P and Helassa, Nordine ORCID: 0000-0003-3743-1886 (2022) Lysosome exocytosis is required for mitosis in mammalian cells. Biochemical and Biophysical Research Communications, 626. pp. 211-219.

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2022.08.024

Abstract

Mitosis, the accurate segregation of duplicated genetic material into what will become two new daughter cells, is accompanied by extensive membrane remodelling and membrane trafficking activities. Early in mitosis, adherent cells partially detach from the substratum, round up and their surface area decreases. This likely results from an endocytic uptake of plasma membrane material. As cells enter cytokinesis they re-adhere, flatten and exhibit an associated increase in surface area. The identity of the membrane donor for this phase of mitosis remains unclear. In this paper we demonstrate how lysosomes dynamically redistribute during mitosis and exocytose. Antagonism of lysosomal exocytosis by pharmacological and genetic approaches causes mitosis failure in a significant proportion of cells. We speculate that either lysosomal membrane or luminal content release, possibly both, are therefore required for normal mitosis progression. These findings are important as they reveal a new process required for successful cell division.

Item Type:	Article
Uncontrolled Keywords:	Exocytosis, Lysosome, Mitosis, Phosphoinositide, TIRFM
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Faculty Management Office Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	22 Aug 2022 09:19
Last Modified:	18 Jan 2023 20:47
DOI:	10.1016/j.bbrc.2022.08.024
Open Access URL:	https://doi.org/10.1016/j.bbrc.2022.08.024
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3161964