Munro, Lachlan Jake and Kell, Douglas B 
ORCID: 0000-0001-5838-7963
(2022)
Analysis of a Library of Escherichia coli Transporter Knockout Strains to Identify Transport Pathways of Antibiotics.
ANTIBIOTICS-BASEL, 11 (8).
1129-.
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Analysis of a Library of iEscherichia colii Transporter Knockout Strains to Identify Transport Pathways of Antibiotics.pdf - Published version Download (3MB) | Preview |
Abstract
Antibiotic resistance is a major global healthcare issue. Antibiotic compounds cross the bacterial cell membrane via membrane transporters, and a major mechanism of antibiotic resistance is through modification of the membrane transporters to increase the efflux or reduce the influx of antibiotics. Targeting these transporters is a potential avenue to combat antibiotic resistance. In this study, we used an automated screening pipeline to evaluate the growth of a library of 447 <i>Escherichia coli</i> transporter knockout strains exposed to sub-inhibitory concentrations of 18 diverse antimicrobials. We found numerous knockout strains that showed more resistant or sensitive phenotypes to specific antimicrobials, suggestive of transport pathways. We highlight several specific drug-transporter interactions that we identified and provide the full dataset, which will be a useful resource in further research on antimicrobial transport pathways. Overall, we determined that transporters are involved in modulating the efficacy of almost all the antimicrobial compounds tested and can, thus, play a major role in the development of antimicrobial resistance.
| Item Type: | Article |
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| Uncontrolled Keywords: | antibiotics, Escherichia coli, transporters |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 12 Oct 2022 08:23 |
| Last Modified: | 18 Jan 2023 20:36 |
| DOI: | 10.3390/antibiotics11081129 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3165388 |
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