Duerst, Celine D, Wiegert, J Simon, Schulze, Christian, Helassa, Nordine 
ORCID: 0000-0003-3743-1886, Toeroek, Katalin and Oertner, Thomas G
(2022)
Vesicular release probability sets the strength of individual Schaffer collateral synapses.
NATURE COMMUNICATIONS, 13 (1).
6126-.
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Abstract
Information processing in the brain is controlled by quantal release of neurotransmitters, a tightly regulated process. From ultrastructural analysis, it is known that presynaptic boutons along single axons differ in the number of vesicles docked at the active zone. It is not clear whether the probability of these vesicles to get released (p<sub>ves</sub>) is homogenous or also varies between individual boutons. Here, we optically measure evoked transmitter release at individual Schaffer collateral synapses at different calcium concentrations, using the genetically encoded glutamate sensor iGluSnFR. Fitting a binomial model to measured response amplitude distributions allowed us to extract the quantal parameters N, p<sub>ves</sub>, and q. We find that Schaffer collateral boutons typically release single vesicles under low p<sub>ves</sub> conditions and switch to multivesicular release in high calcium saline. The potency of individual boutons is highly correlated with their vesicular release probability while the number of releasable vesicles affects synaptic output only under high p<sub>ves</sub> conditions.
| Item Type: | Article |
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| Uncontrolled Keywords: | Hippocampus, Presynaptic Terminals, Synapses, Synaptic Vesicles, Calcium, Glutamic Acid, Neurotransmitter Agents, Probability, Synaptic Transmission |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 25 Oct 2022 07:41 |
| Last Modified: | 18 Jan 2023 19:49 |
| DOI: | 10.1038/s41467-022-33565-6 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3165738 |
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