Al-Maslamani, Noor A, Oldershaw, Rachel 
ORCID: 0000-0001-8478-599X, Tew, Simon, Curran, Jude, D'Hooghe, Pieter, Yamamoto, Kazuhiro ORCID: 0000-0002-8481-775X and Horn, Henning F
(2022)
Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations.
CELLS, 11 (24).
4011-.
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Abstract
Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required for implantation; however, during the expansion these cells de-differentiate and lose their chondrocyte phenotype. In this review we focus on examining the de-differentiation phenotype from a mechanobiology and biophysical perspective, highlighting some of the nuclear mechanics and chromatin changes in chondrocytes seen during the expansion process and how this relates to the gene expression profile. We propose that manipulating chondrocyte nuclear architecture and chromatin organization will highlight mechanisms that will help to preserve the chondrocyte phenotype.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | autologous chondrocyte implantation, de-differentiation, re-differentiation, chondrocyte, mechanobiology, biophysics, two-dimensional culture (2D), three-dimensional culture (3D) |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 12 Dec 2022 12:38 |
| Last Modified: | 18 Jan 2023 19:39 |
| DOI: | 10.3390/cells11244011 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166611 |
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