Cyanostilbene-based fluorescent paper array for monitoring fish and meat freshness via amino content detection

Dhinakaran, Manivannan Kalavathi, Smith, Barry Lee ORCID: 0000-0001-5571-3647, Vilaivan, Tirayut, Maher, Simon and Praneenararat, Thanit (2023) Cyanostilbene-based fluorescent paper array for monitoring fish and meat freshness via amino content detection. Microchimica Acta, 190 (6). 215-.

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Abstract

The detection of biogenic amines released from degraded meats is an effective method for evaluating meat freshness. However, existing traditional methods like titration are deemed tedious, while the use of sophisticated analytical instruments is not amenable to field testing. Herein, a cyanostilbene-based fluorescent array was rapidly fabricated using macroarray synthesis on a cellulose paper surface to detect amines liberated from spoiled beef, fish, and chicken. The fluorescence changes of immobilized molecules from the interaction with gaseous amines were used to monitor changes in freshness levels. Thanks to the high-throughput nature of macroarray synthesis, a set of highly responsive molecules such as pyridinium and dicyanovinyl moieties were quickly revealed. Importantly, this method offers flexibility in sensing applications including (1) sensing by individual sensor molecules, where the fluorescence response correlated well with established titration methods, and (2) collective sensing whereby chemometric analysis was used to provide a cutoff of freshness with 73-100% accuracy depending on meat types. Overall, this study paves the way for a robust and cost-effective tool for monitoring meat freshness.

Item Type:	Article
Uncontrolled Keywords:	Food spoilage, Biogenic amines, Sensor array, Paper-based sensor, Optoelectronic nose
Divisions:	Faculty of Science and Engineering > School of Electrical Engineering, Electronics and Computer Science
Depositing User:	Symplectic Admin
Date Deposited:	15 May 2023 07:36
Last Modified:	28 Jun 2023 12:47
DOI:	10.1007/s00604-023-05787-y
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3170323