Sposito, Francesca ORCID: 0009-0007-0272-0318, Northey, Sarah, Charras, Amandine ORCID: 0000-0001-6760-5459, McNamara, Paul S ORCID: 0000-0002-7055-6034 and Hedrich, Christian M ORCID: 0000-0002-1295-6179
(2023)
Hypertonic saline induces inflammation in human macrophages through the NLRP1 inflammasome.
Genes and immunity, 24 (5).
pp. 263-269.
Abstract
Nebulized hypertonic saline (3-7%) is commonly used to increase mucociliary clearance in patients with chronic airway disease and/or virus infections. However, altered salt concentrations may contribute to inflammatory responses. The aim of this study was to investigate whether 500 mM NaCl (3%) triggers inflammation in human macrophages and identify the molecular mechanisms involved. NaCl-induced pyroptosis, IL-1β, IL-18 and ASC speck release were measured in primary human monocyte-derived macrophages. Treatment with the recombinant IL-1 receptor antagonist anakinra or the NLRP3 inhibitor MCC950 did not affect NaCl-mediated inflammasome assembly. Knock-down of NLRP1 expression, but not of NLRP3 and NLRC4, reduced NaCl-induced pyroptosis, pro-inflammatory cytokine and ASC speck release from human THP-1-derived macrophages. Data from this study suggest that 3% NaCl-induced inflammatory responses in human macrophages depend on NLRP1 and inflammasome assembly. Targeting inflammation in addition to inhalation with hypertonic saline may benefit patients with inflammatory airway disease.
Item Type: | Article |
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Uncontrolled Keywords: | Macrophages, Humans, Inflammation, Sodium Chloride, Interleukin-1beta, Inflammasomes, NLR Family, Pyrin Domain-Containing 3 Protein, NLR Proteins |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
Depositing User: | Symplectic Admin |
Date Deposited: | 22 Aug 2023 09:43 |
Last Modified: | 09 Dec 2023 02:10 |
DOI: | 10.1038/s41435-023-00218-7 |
Open Access URL: | https://doi.org/10.1038/s41435-023-00218-7 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3172288 |