Aminosilane Functionalized Aligned Fiber PCL Scaffolds for Peripheral Nerve Repair

Taylor, Caroline S, Barnes, Joseph, Koduri, Manohar Prasad, Haq, Shamsal, Gregory, David A, Roy, Ipsita, D'Sa, Raechelle A ORCID: 0000-0003-2651-8783, Curran, Judith ORCID: 0000-0003-1551-2917 and Haycock, John W (2023) Aminosilane Functionalized Aligned Fiber PCL Scaffolds for Peripheral Nerve Repair. MACROMOLECULAR BIOSCIENCE, 23 (11). e2300226-.

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Official URL: http://dx.doi.org/10.1002/mabi.202300226

Abstract

Silane modification is a simple and cost-effective tool to modify existing biomaterials for tissue engineering applications. Aminosilane layer deposition has previously been shown to control NG108-15 neuronal cell and primary Schwann cell adhesion and differentiation by controlling deposition of ─NH<sub>2</sub> groups at the submicron scale across the entirety of a surface by varying silane chain length. This is the first study toreport depositing 11-aminoundecyltriethoxysilane (CL11) onto aligned Polycaprolactone (PCL) scaffolds for peripheral nerve regeneration. Fibers are manufactured via electrospinning and characterized using water contact angle measurements, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). Confirmed modified fibers are investigated using in vitro cell culture of NG108-15 neuronal cells and primary Schwann cells to determine cell viability, cell differentiation, and phenotype. CL11-modified fibers significantly support NG108-15 neuronal cell and Schwann cell viability. NG108-15 neuronal cell differentiation maintains Schwann cell phenotype compared to unmodified PCL fiber scaffolds. 3D ex vivo culture of Dorsal root ganglion explants (DRGs) confirms further Schwann cell migration and longer neurite outgrowth from DRG explants cultured on CL11 fiber scaffolds compared to unmodified scaffolds. Thus, a reproducible and cost-effective tool is reported to modify biomaterials with functional amine groups that can significantly improve nerve guidance devices and enhance nerve regeneration.

Item Type:	Article
Uncontrolled Keywords:	aminosilanes, electrospun fibers, nerve regeneration, peripheral nerves, silane modification, topographical guidance
Divisions:	Faculty of Science and Engineering > School of Engineering
Depositing User:	Symplectic Admin
Date Deposited:	22 Sep 2023 15:40
Last Modified:	05 Feb 2024 10:41
DOI:	10.1002/mabi.202300226
Open Access URL:	https://doi.org/10.1002/mabi.202300226
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3172982