Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung.

Hernandez Pichardo, Alejandra ORCID: 0000-0001-7774-4452, Wilm, Bettina ORCID: 0000-0002-9245-993X, Liptrott, Neill J ORCID: 0000-0002-5980-8966 and Murray, Patricia ORCID: 0000-0003-1316-148X (2023) Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung. Stem cells international, 2023. p. 7397819.

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Official URL: http://dx.doi.org/10.1155/2023/7397819

Abstract

Mesenchymal stromal cells (MSCs) administered intravenously (IV) have shown efficacy in preclinical models of various diseases. This is despite the cells not reaching the site of injury due to entrapment in the lungs. The immunomodulatory properties of MSCs are thought to underlie their therapeutic effects, irrespective of whether they are sourced from bone marrow, adipose tissue, or umbilical cord. To better understand how MSCs affect innate immune cell populations in the lung, we evaluated the distribution and phenotype of neutrophils, monocytes, and macrophages by flow cytometry and histological analyses after delivering human umbilical cord-derived MSCs (hUC-MSCs) IV into immunocompetent mice. After 2 hr, we observed a significant increase in neutrophils, and proinflammatory monocytes and macrophages. Moreover, these immune cells localized in close proximity to the MSCs, suggesting an active role in their clearance. By 24 hr, we detected an increase in anti-inflammatory monocytes and macrophages. These results suggest that the IV injection of hUC-MSCs leads to an initial inflammatory phase in the lung shortly after injection, followed by a resolution phase 24 hr later.

Item Type:	Article
Uncontrolled Keywords:	Lung, Stem Cell Research - Nonembryonic - Human, Stem Cell Research - Umbilical Cord Blood/ Placenta, Regenerative Medicine, Stem Cell Research, Stem Cell Research - Umbilical Cord Blood/ Placenta - Human, 2.1 Biological and endogenous factors, 2 Aetiology, Inflammatory and immune system
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	29 Sep 2023 09:35
Last Modified:	14 Mar 2024 20:09
DOI:	10.1155/2023/7397819
Open Access URL:	https://doi.org/10.1155/2023/7397819
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3173206