Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation.

Shakir, Dilem, Batie, Michael ORCID: 0000-0002-7508-6641 and Rocha, Sonia (2023) Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation. Methods in molecular biology (Clifton, N.J.), 2675. pp. 261-269.

PDF Imaging_methods Shakir.pdf - Author Accepted Manuscript Download (177kB) | Preview

Abstract

Hypoxia and its signalling pathway play a key role in human physiology and a variety of diseases. Alterations in histone methylation coordinate transcriptional responses to hypoxia. Here, we detail a fixed cell immunofluorescence method for quantifying hypoxia-induced changes in histone methylation, exemplified by the measurement of H3K27me3.

Item Type:	Article
Uncontrolled Keywords:	Humans, Histones, Fluorescent Antibody Technique, DNA Methylation, Protein Processing, Post-Translational, Methylation, Hypoxia
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	29 Sep 2023 09:41
Last Modified:	29 Sep 2023 09:41
DOI:	10.1007/978-1-0716-3247-5_19
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3173208